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Genome Research
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License: CC BY NC
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Europe PubMed Central
Other literature type . 1999
Data sources: PubMed Central
Hyper Article en Ligne
Other literature type . 1999
Genome Research
Article . 1999 . Peer-reviewed
Data sources: Crossref
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Finding New Human Minisatellite Sequences in the Vicinity of Long CA-Rich Sequences

Authors: Giraudeau, F; Petit, E; Avet-Loiseau, H; Hauck, Y; Vergnaud, Gilles; Amarger, V;

Finding New Human Minisatellite Sequences in the Vicinity of Long CA-Rich Sequences

Abstract

Microsatellites and minisatellites are two classes of tandem repeat sequences differing in their size, mutation processes, and chromosomal distribution. The boundary between the two classes is not defined. We have developed a convenient, hybridization-based human library screening procedure able to detect long CA-rich sequences. Analysis of cosmid clones derived from a chromosome 1 library show that cross-hybridizing sequences tested are imperfect CA-rich sequences, some of them showing a minisatellite organization. All but one of the 13 positive chromosome 1 clones studied are localized in chromosomal bands to which minisatellites have previously been assigned, such as the 1pter cluster. To test the applicability of the procedure to minisatellite detection on a larger scale, we then used a large-insert whole-genome PAC library. Altogether, 22 new minisatellites have been identified in positive PAC and cosmid clones and 20 of them are telomeric. Among the 42 positive PAC clones localized within the human genome by FISH and/or linkage analysis, 25 (60%) are assigned to a terminal band of the karyotype, 4 (9%) are juxtacentromeric, and 13 (31%) are interstitial. The localization of at least two of the interstitial PAC clones corresponds to previously characterized minisatellite-containing regions and/or ancestrally telomeric bands, in agreement with this minisatellite-like distribution. The data obtained are in close agreement with the parallel investigation of human genome sequence data and suggest that long human (CA)s are imperfect CA repeats belonging to the minisatellite class of sequences. This approach provides a new tool to efficiently target genomic clones originating from subtelomeric domains, from which minisatellite sequences can readily be obtained.[The sequence data described in this paper have been submitted to the EMBL data library under accession nos.AJ000377–AJ000383.]

Country
France
Subjects by Vocabulary

Microsoft Academic Graph classification: Minisatellite Repeat Biology Tandem repeat Genomic library Genetics Hybridization probe Minisatellite Cosmid Microsatellite Human genome

Keywords

Letter, Databases, Factual, [SDV]Life Sciences [q-bio], Molecular Sequence Data, Minisatellite Repeats, [SDV.GEN] Life Sciences [q-bio]/Genetics, Genetics, Humans, Bacteriophage P1, Dinucleotide Repeats, In Situ Hybridization, Fluorescence, Genetics (clinical), Genomic Library, [SDV.GEN]Life Sciences [q-bio]/Genetics, Chromosome Mapping, DNA, Sequence Analysis, DNA, Cosmids, [SDV] Life Sciences [q-bio], DNA Probes

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  • citations
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    7
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    Average
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    Average
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    Average
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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
7
Average
Average
Average
Green
hybrid
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Neuroinformatics
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