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Blood
Article
Data sources: UnpayWall
Blood
Article . 1997 . Peer-reviewed
Data sources: Crossref
Blood
Article . 1997
Hal-Diderot
Other literature type . 1997
Data sources: Hal-Diderot
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Sorting of the Specific Granule Protein, NGAL, During Granulocytic Maturation of HL-60 Cells

Authors: V, Le Cabec; J, Calafat; N, Borregaard;

Sorting of the Specific Granule Protein, NGAL, During Granulocytic Maturation of HL-60 Cells

Abstract

AbstractThe different types of human neutrophil granules (azurophil, specific, and gelatinase granules) are formed sequentially during maturation of neutrophils from the promyelocyte stage to the band cell stage. The promyelocytic HL-60 cells can maturate to segmented granulocytes but are incapable of activating the transcription of any known intragranular protein, normally located in specific or gelatinase granules. To study the sorting of granule proteins during maturation, we transfected HL-60 cells with the specific granule protein NGAL, inserted under control of a cytomegalovirus promoter. We previously showed that NGAL is sorted to azurophil granules and colocalizes with myeloperoxidase in undifferentiated HL-60 cells. We show here that, when such transfected HL-60 cells differentiate into granulocytes, newly synthesized NGAL is not retained in granules but is constitutively secreted. This indicates that highly specific mechanisms must exist that are responsible for diverting transport vesicles into storage granules, and that HL-60 cells not only lack the ability to activate transcription of specific granule proteins, but also lose the ability to form storage granules during maturation.

Country
France
Subjects by Vocabulary

Microsoft Academic Graph classification: Cellular differentiation Granulocyte Azurophilic granule medicine biology Granule (cell biology) Degranulation Cell biology medicine.anatomical_structure Specific granule Myeloperoxidase biology.protein Promyelocyte

Keywords

MESH: Cell Differentiation, Immunology, HL-60 Cells, MESH: Carrier Proteins, MESH: Cytoplasmic Granules, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Cytoplasmic Granules, Transfection, Biochemistry, MESH: Oncogene Proteins, Lipocalin-2, MESH: HL-60 Cells, MESH: Peroxidase, Proto-Oncogene Proteins, Humans, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, Peroxidase, Oncogene Proteins, MESH: Humans, MESH: Transfection, Cell Differentiation, Cell Biology, Hematology, Lipocalins, MESH: Proto-Oncogene Proteins, MESH: Granulocytes, MESH: Acute-Phase Proteins, Carrier Proteins, Acute-Phase Proteins, Granulocytes

43 references, page 1 of 5

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4. Kjeldsen L, Bainton DF, Sengeløv H, Borregaard N: Structural and functional heterogeneity among peroxidase-negative granules in human neutrophils: Identification of a distinct gelatinase containing granule subset by combined immunocytochemistry and subcellular fractionation. Blood 82:3183, 1993 [OpenAIRE]

5. Dewald B, Bretz U, Baggiolini M: Release of gelatinase from a novel secretory compartment of human neutrophils. J Clin Invest 70:518, 1982 [OpenAIRE]

6. Sengeløv H, Kjeldsen L, Borregaard N: Control of exocytosis in early neutrophil activation. J Immunol 150:1535, 1993 [OpenAIRE]

7. Sengeløv H, Follin P, Kjeldsen L, Lollike K, Dahlgren C, Borregaard N: Mobilization of granules and secretory vesicles during in vivo exudation of human neutrophils. J Immunol 154:4157, 1995 [OpenAIRE]

8. Borregaard N, Sehested M, Nielsen BS, Sengeløv H, Kjeldsen L: Biosynthesis of granule proteins in normal human bone marrow cells. Gelatinase is a marker of terminal neutrophil differentiation. Blood 85:812, 1995 [OpenAIRE]

9. Borregaard N: Current opinion about neutrophil granule physiology. Cur Opin Hematol 3:11, 1996 [OpenAIRE]

10. Bainton DF: Neutrophil granules. Br J Haematol 29:17, 1975 [OpenAIRE]

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  • citations
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    62
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    Top 10%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    Top 10%
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    Top 10%
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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
62
Top 10%
Top 10%
Top 10%
bronze
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Neuroinformatics
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