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Effects of recombinant endo-N overproduction on K1-specific phage sensitivity

Authors: Steenbergen, Susan M; Vimr, Eric R;

Effects of recombinant endo-N overproduction on K1-specific phage sensitivity

Abstract

Bacteria were grown to early stationary phase with appropriate drugs and IPTG added where indicated to a final concentration of 1 mM to induce endo-N production when cells reached an A of 0.3. Bacteria (0.15 ml) were plated with approximately 85 K1F plaque forming units in top agar on LB and incubated at 37°C overnight prior to photography. A. Acapsular mutant EV93. B. EV36 wild type. C. EV36 harbouring pRep4 and pEndo-N, uninduced. D. EV36 harbouring pRep4 and pEndo-N, induced and plated to LB containing 1 mM IPTG.Copyright information:Taken from "Biosynthesis of the K1 group 2 polysialic acid capsule occurs within a protected cytoplasmic compartment"Molecular Microbiology 2008;68(5):1252-1267.Published online Jan 2008PMCID:PMC2408645.© 2008 The Authors. Journal compilation © 2008 Blackwell Publishing Ltd

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  • citations
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    0
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    Average
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    Average
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    Average
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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average